DescriptionThe HCT-8 line is identical to the HRT-18 cell line; the cells are positive for keratin by immunoperoxidase staining.Tumorigenecity: yes, in nude mice
Isoenzyme: AK-1, 1; ES-D, 1-2; GLO-1, 2; G6PD, B; PGM1, 1; PGM3, 1; Me-2, 1
Histopathology: adenocarcinoma
Reverse transcritase: Production
Organism: Homo sapiens, human
STR DNA Profile: D3S1358: 17
vWA: 18,19
FGA: 22
Amelogenin: X,Y
TH01: 7,9.3
TPOX: 8,11
CSF1P0: 12
D5S818: 13
D13S317: 8,11
D7S820: 10,12
Karyotype: 2n = 46, pseudodiploid
Source: ileum
Culture Properties: adherent
Morphology: epithelial
Recommended Medium: RPMI-1640 + 2 mM L-glutamine + 1 mM sodium pyruvate+ 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% FBS
Quality Control: Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping: liquid nitrogen vapor phase
Disease: colon adenocarcinoma
Application: This cell line is used for tumourigenicity studies.